Blar i Publikasjoner fra Cristin - NILU på tidsskrift "Frontiers in Toxicology"

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    • The alamar blue assay in the context of safety testing of nanomaterials 

      Longhin, Eleonora Marta; El Yamani, Naouale; Rundén-Pran, Elise; Dusinska, Maria (Peer reviewed; Journal article, 2022)
      The Alamar Blue (AB) assay is widely used to investigate cytotoxicity, cell proliferation and cellular metabolic activity within different fields of toxicology. The use of the assay with nanomaterials (NMs) entails specific ...

    • The colony forming efficiency assay for toxicity testing of nanomaterials – Modifications for higher-throughput 

      Rundén-Pran, Elise; Mariussen, Espen; El Yamani, Naouale; Elje, Elisabeth; Longhin, Eleonora Marta (Peer reviewed; Journal article, 2022)
      To cope with the high number of nanomaterials manufactured, it is essential to develop high-throughput methods for in vitro toxicity screening. At the same time, the issue with interference of the nanomaterial (NM) with ...

    • Common Considerations for Genotoxicity Assessment of Nanomaterials 

      Elespuru, Rosalie K.; Doak, Shareen H.; Collins, Andrew Richard; Dusinska, Maria; Pfuhler, Stefan; Manjanatha, Mugamane; Cardoso, Renato M.S.; Chen, Connie L. (Peer reviewed; Journal article, 2022)
      Genotoxicity testing is performed to determine potential hazard of a chemical or agent for direct or indirect DNA interaction. Testing may be a surrogate for assessment of heritable genetic risk or carcinogenic risk. Testing ...

    • In vivo Mammalian Alkaline Comet Assay: Method Adapted for Genotoxicity Assessment of Nanomaterials 

      Cardoso, Renato; Dusinska, Maria; Collins, Andrew Richard; Manjanatha, Mugamane; Pfuhler, Stefan; Registre, Marilyn; Elespuru, Rosalie K. (Peer reviewed; Journal article, 2022)
      The in vivo Comet assay measures the generation of DNA strand breaks under conditions in which the DNA will unwind and migrate to the anode in an electrophoresis assay, producing comet-like figures. Measurements are on ...

    • The miniaturized enzyme-modified comet assay for genotoxicity testing of nanomaterials 

      El Yamani, Naouale; Rundén-Pran, Elise; Collins, Andrew Richard; Longhin, Eleonora Marta; Elje, Elisabeth; Hoet, Peter; Vrček, Ivana Vinković; Doak, Shareen H.; Fessard, Valérie; Dusinska, Maria (Peer reviewed; Journal article, 2022)
      The in vitro comet assay is a widely applied method for investigating genotoxicity of chemicals including engineered nanomaterials (NMs). A big challenge in hazard assessment of NMs is possible interference between the NMs ...

    • New approach methodologies to facilitate and improve the hazard assessment of non-genotoxic carcinogens—a PARC project 

      Audebert, Marc; Assmann, Ann-Sophie; Azqueta, Amaya; Babica, Pavel; Benfenati, Emilio; Bortoli, Sylvie; Bouwman, Peter; Braeuning, Albert; Burgdorf, Tanja; Coumoul, Xavier; Debizet, Kloé; Dusinska, Maria (Peer reviewed; Journal article, 2023)
      Carcinogenic chemicals, or their metabolites, can be classified as genotoxic or non-genotoxic carcinogens (NGTxCs). Genotoxic compounds induce DNA damage, which can be detected by an established in vitro and in vivo battery ...

    • Thymidine Kinase+/− Mammalian Cell Mutagenicity Assays for Assessment of Nanomaterials 

      Chen, Tao; Dusinska, Maria; Elespuru, Rosalie K. (Peer reviewed; Journal article, 2022)
      The methods outlined here are part of a series of papers designed specifically for genotoxicity assessment of nanomaterials (NM). Common Considerations such as NM characterization, sample preparation and dose selection, ...